Plasmid dna purification using the qiaprep spin miniprep kit and a microcentrifuge. Qiaprep spin qiaprep miniprep handbook 052004 23 protocol. For purification of lowcopy plasmids and cosmids, large plasmids 110 kbl, and dna prepared using. Grow yeast strain containing desired plasmid overnight in 10 ml of rich. Qiaprep spin miniprep kit protocol for yeast using a microcentrifuge.
Technology silicabased spinvacuum column anionexchange gravityflow column silicabased spinvacuum plates. Plasmid dna purification using qiaprep spin miniprep kit. The innovative qiacube uses advanced technology to process qiagen spin columns, enabling. Qiagen qiaprep spin miniprep kit, 50 preps, 1 to 100ml culture volume, 50l elution volume, molecular biology grade plasmid dna purification, silica technology, spin column format, manual processing.
The qiaprep spin miniprep kit can be fully automated on the qiacube. The isolated dna is acceptable for restriction digests, ligation, and other subcloning applications. Qiagen plasmid purification handbook harvard university. Apr 28, 2014 the classic qiagen bindwashwashelute protocol for the qiaprep spin miniprep kit involves several centrifugation steps. Qiaquick 96 pcr purification kit 4x96 qiaquick 96 pcr purification kit 24x96 pcr9604. Qiagen qiaprep 96 turbo miniprep kit, 24 x 96 preps, 1 to 10ml culture volume, molecular biology grade plasmid dna purification, silica technology, manual processing, 48 to 96 samplesrun, 20g yield, for purification of up to 20g molecular biology. For purification of up to 20 micro g molecular biology grade plasmid dna per well. Qiagen qiastock pricing prices effective sept 1 2017. Qiaprep spin miniprep kit using a microcentrifuge 23 using 5 ml collection tubes 24 using a vacuum manifold 25 qiaprep 8 miniprep kit 28 qiaprep 8 turbo miniprep kit 31 qiaprep 96 turbo miniprep kit 33 cell cultivation in a 96well block 36 special applications 37 troubleshooting guide 38 appendix a. Qiaprep spin miniprep kit from qiagen biocompare product. Qiaprep spin miniprep kit protocol for yeast using a microcentrifuge procedure 1.
These are available online in convenient and compact pdf format at. Timerun, 20g yield, ideal for fluorescent and radioactive sequencing. For purification of up to 20 micro g molecular biology grade plasmid dna. Qiaprep spin miniprep kit for purification of highquality dna. Qiaprep 96 turbo miniprep kit 24, 27193 from qiagen. Qiaprep miniprep handbook 052012 5 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. Check on qiacube web portal for most recent protocol. Nucleospin plasmid no lid support protocol for the qiacube. Qiaprep miniprep handbook 052012 3 contents kit contents 4 storage 5 intended use 5 safety information 5 quality control 6 introduction 7 principle 8 using lyseblue reagent 11 important notes 12 guidelines for qiavac manifolds 15 protocols plasmid dna purification using the qiaprep spin miniprep kit and a microcentrifuge 19 and 5 ml collection. Transfer 250ul of affinity gel hag to spin column in a 2ml collection tube. If you have never done this protocol before, read through the manual that comes with the qiagen spin miniprep kit. University of guelph biobar 2020 price list jan 1 through dec. Qiaprep spin midimaxiprep kit protocol before staring make sure that rnase a has been added to p1 buffer. Purifying plasmid dna using the qiagen miniprep kit protocol.
Qiaprep spin miniprep kit 250 from qiagen sample to insight. Genelute hp 96 well plasmid miniprep kit 4x96 pcr cleanup. Kit contents qiaprep spin miniprep kit 50 250 catalog no. Qiaprep spin miniprep kit 50, 27104 from qiagen labsave. Nucleopore hisspin protein miniprep genetix biotech. Plasmid dna purification using the qiaprep spin miniprep kit with. For more information, please refer to the qiaprep miniprep handbook, december. Washthecolumnwith4resinvolumeofdenaturingbindingbufferbyresuspendingtheresin androckingfortwominutes. Qiaprep spin columns can be used in a microcentrifuge or on any vacuum manifold with luer connectors e. Qiaprep spin miniprep kit 250 from qiagen sample to. Plasmid dna samples that are free of rna and genomic dna contamination, that lack any. With the genelute kits, mrnabead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mm trishcl, ph 7.
Qiagen qiaprep spin miniprep kit, 250 preps, 1 to 100ml culture volume. Cross reference of genelute to qiagen nucleic acid. Qiagen is the leading provider of innovative sample and assay technologies, enabling plasmid dna purification using the qiaprep spin miniprep kit. The protocol allowed for higher yield by reducing the. The classic qiagen bindwashwashelute protocol for the qiaprep spin miniprep kit involves several centrifugation steps. Plasmid dna purification using qiaprep spin miniprep kit and. Qiagen qiastock pricing prices effective sept 1 2017 through.
Buffer composition for miniprep kit molecular biology. Qiaprep spin miniprep kit has the efficiency of a reproducible assay along with an ease of procedure. Plasmid dna purification using the qiaprep spin miniprep kit and a. Kits can be stored for up to 12 months without showing any reduction in performance and quality. Qiaprep spin miniprep kit 250 from qiagen selectscience. Pellet cells in 15 ml falcon tube, resuspend in water, and transfer to 1.
Four different cell lines cos7, hek293, hela, and huh7 were grown to 8090% confluence and transfected with 100 ng of each plasmid, in complex with 0. Plasmid dna purification using the qiaprep spin miniprep kit and a microcentrifuge this protocol is desig ned for purification of up to 20 pg of h ighcopy plasmid dna from 15 ml overnight cultures of e coli in lb luriobertanil medium. Dna purification and analysis maximize sample yield, purity and integrity. Aug 02, 2016 the classic qiagen bindwashwashelute protocol for the qiaprep spin miniprep kit involves several centrifugation steps. Genelute hp plasmid miniprep kit 70 genelute plasmid miniprep kit 70 27106.
Qiaprep spin miniprep kit highyield protocol english pdf. New england biolabs uk ltd monarch plasmid miniprep kit. Qiaprep spin miniprep kit 50 from qiagen sample to insight. Qiacube instruments are preinstalled with protocols f or purification of plasmid dna, genomic dna, rna, viral nucleic acids and proteins, plus dna and rna cleanup. Plasmid dna purification using the qiaprep spin miniprep kit and a microcentrifuge this protocol is designed for purification of up to 20 g of highcopy plasmid dna from 15 ml overnight cultures of e. Here at igem we use qiagen spin miniprep kits for doing small batches of minipreps. Please read important notes on pages 11 before starting. Qiagen qiaprep spin miniprep kit, 250 preps, 1 to 100ml culture volume, 50l elution volume, molecular biology grade plasmid dna purification, silica technology, spin column format, manual processing, protocol. Grow yeast strain containing desired plasmid overnight in 10 ml of rich media yepd.
This represents a time saving of 50% compared with qiaprep spin miniprep kit. Purelink quick plasmid miniprep kit purelink hipure plasmid miniprep kit purelink pro quick96 plasmid kit magjet plasmid dna. Qiagen qiaprep spin miniprep kit, 250 preps, 1 to 100ml culture volume, 50l elution volume, molecular biology grade plasmid dna purification, silica technology, spin column format, manual processing. The most difficult buffer for miniprep is the neutralization buffer columns. Qiaprep 96 turbo miniprep kit 24, 27193 from qiagen labsave. Aug 03, 2016 the final protocol for large scale mirapreps was tested with genejet, qiagen, and sigma genelute spin column miniprep kits, using the resuspension, lysis, neutralization and wash buffers provided a stepby step protocol is provided in s1 file. Qiaprep spin miniprep kit using a microcentrifuge this protocol is designed for purification of up to 20 g of highcopy plasmid dna from 15 ml overnight cultures of e. We have had good success with this kit the instructions are very easy to follow and all the necessary reagents are supplied buffer p1 must be kept at 4. Purifying plasmid dna from bacterial colonies using the qiagen miniprep kit. Plasmid dna encoding constitutively expressed gfp pegfpc2 was prepared using either monarch plasmid miniprep kit or qiagen qiaprep spin miniprep kit. Plasmid dna purification using the qiaprep spin miniprep kit and a microcentrifuge the idea a miniprep is a way of extracting plasmid dna from cells. Dna purification and analysis thermo fisher scientific us. The following has been reproduced from their protocol and annotated based on experience with the kit.
Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. Qiaprep miniprep handbook 112005 7 storage qiaprep miniprep kits should be stored dry at room temperature 1525c. One kit for all applications comparisons on this page based on internal data. We have experience using the qiaprep spin miniprep kit, which can be used to process 120 samples in about 3045 minutes. Qiaprep spin miniprep kit highyield protocol english pdf print bookmark share for purification of up to 30. Qiagen qiaprep spin miniprep kit, 50 preps, 1 to 100ml culture volume, 50l elution volume, molecular biology grade plasmid dna purification, silica technology, spin column format, manual processing, 30 min. Qiaprep 96 turbo miniprep kit 4x96 qiaprep 96 turbo miniprep kit 24x96 na9604. Instead, the plasmid dna is precipitated with isopropanol. Qiagen qiaprep spin miniprep kit, 50 preps, 1 to 100ml culture volume. Qiaprep spin miniprep kit using a microcentrifuge this protocol is designed for purification of up to 20.
Genelute 96 well pcr cleanup kit 4x96 dna purification. For purification of up to 20 g molecular biology grade plasmid dna. University of guelph biobar 2020 price list jan 1 through. The pureyield plasmid miniprep system is designed to purify 1. Plasmid dna purification using the quicklyse miniprep kit this protocol is designed for purification of 38 g highcopy plasmid dna from 1. While guaranteeing excellent flowthrough of all the wash buffers and. Kits can be stored for up to 12 months without showing any reduction in performance and qual ity.
1551 1117 1332 542 797 1220 816 649 163 214 1304 1092 372 550 376 455 1036 992 1262 199 1483 220 1518 310 464 1375 205 201 143 883 1392 426 1212 815 1491 267 1282 30 273 1025 47 978