The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The 1 kb plus dna ladder for safe stains might be used on sybr green i precast agarose gels but might require further dilution and optimization. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Gel loading buffer timing varies depending on amplicon length selection guide pcr enzymes and master mixes go online to view related products. How can i prepare working solution with 1 kb dna ladder supplied by promega. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. A molecularweight size marker in the form of a 1kb dna ladder in the rightmost lane, used in gel electrophoresis. The 1 kb plus dna ladder consists of 18 individual chromatographypurified dna fragments ranging in size from 100 bp to 15,000 bp. Free sample available, use link below to request free. Directload 1 kb dna ladder readytouse marker for dna. Quickload purple 100 bp dna ladder is a readytoload marker ranging from 1001517 bp.
A reference band at 1,500 bp is included for easy orientation. Fullranger 100bp dna ladder 100bp 5000bp good range for small and large cloning applications. Catalog number size important guidelines 10787018 250 g. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp.
Dna ladders have defined sizes, and are not intended for use in quantitative analysis. All yellow highlighted numbers need to line up with the corresponding bands in. Dna marker bp 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis features. So im wondering if maybe the dna ladder ran differently in the gel compared to the rna samples. It allows sizing and concentration estimate of dna fragments on. General description sigmas directload 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. There have been no changes to product formulation or specifications. How can i prepare working solution with 1 kb dna ladder. Gel conditions are 1% agarose, 3 voltcm, and ethidium bromide stain. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. The 1 kb plus dna ladder for safe stains is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Is it ok to use dna molecular weight marker in rna electrophoresis. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen. Nucleic acid ladder is used as a size standard in agarose gel electrophoresis.
Is it ok to use dna molecular weight marker in rna. Generuler 1 kb plus dna ladder thermo fisher scientific. Dna marker bp 1 kb blue is designed for use as molecular weight standard for agarose gel electrophoresis availibility of sample size may be limited. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. The ladder is premixed with gel load buffer color dyes with varying mobility on an. Do not heat the trackit 1kb plus dna ladder before loading. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds. Try the 1 kb plus dna ladder from neb, a single convenient ladder for 100 bp up to 10 kb.
Every readytouse ladder is supplied with the nuclease free loading dye solution, which ensures optimal migration and quantification of your dna probes. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. The accessory proteins reep5 and reep6 refine cxcr1. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick. The 1 kb dna ladder has a number of proprietary plasmids that are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards. Nebs quickload purple dna ladders offer sharp, uniform bands with easily identifiable reference bands. This product is available in quickload purple format enjoy the convenience of a readytoload format. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. Tracking dye does not leave a shadow under uv light. The 1 kb dna ladder is a unique combination of a number of. B qrtpcr was performed for reep5 and reep6 mrna in a549 cells infected with lentivirus. Highranger 1kb dna ladder 300bp 0bp size determination of digested dna. Find and purchase 1 kb plus dna ladder products at invitrogen life science technologies. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment.
The bands of the ladder each contain from 1 to 12 repeats of a 1018. Powerful tool for estimating molecular weight of linear, doublestranded dna fragments. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. Product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable cas. This marker is ideal for the size determination of digested dna. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder.
Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. The invitrogen 1kb plus dna ladder is designed for sizing and quantification of double stranded dna on 0. Download a pdf containing pricing for our full product list. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Sm, size markers 1 kb plus dna ladder from invitrogen. The dna ladder consists of proprietary plasmids which are. For technical questions about this product, call the invitrogen techlinesm u. Find and order marker and products like excelband 1kb plus 0. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double.
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